Top 22 Chemistry Interview Questions and Answers (2023)
Here are the most asked basic to advanced level Chemistry interview questions and answers for freshers as well as experienced candidates to get their dream job.
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1) Explain the term Aliquot and Diluent?
- Aliquot: It is a measured sub-volume of the original sample
- Diluent: Material with which sample is diluted
2) What is molality?
Molality is the number of solutes that are present in 1 kg of a solvent.
3) What is titration?
Titration is a process to determine the molarity of a base or an acid. In this process, a reaction is carried out between the known volumes of a solution with a known concentration, against the known volume of a solution with an unknown concentration.
4) What is a buffer?
A buffer is an aqueous solution which has highly stable pH. It is a blend of a weak acid and its conjugate base or vice versa. On adding a small amount of base or acid to buffer, its pH hardly changes.
5) How does buffer work?
In buffer when hydrogen ion is added, it will be neutralized by the base in the buffer. Hydroxide ion will be neutralized by the acid. On the overall pH of the buffer solution, these neutralization reactions will not show much effect.
While when you select an acid as a buffer solution, try to use an acid that has a pH closed to your desired pH. This will help your buffer to achieve a nearly equivalent amount of acid and conjugate base, so that it will enable to neutralize as much as H+ and OH -.
6) What is a mole?
Mole is the unit used to define the number of chemical substance present in a substance. It is the amount of substance which consists of the same number of chemical units as there are atoms in exactly 12 gram of pure carbon-12.

7) How will you calculate how many moles of glucose present in 320 mL of 5.0 M of glucose solution?
First step: Convert the volume from millilitres to litres
- 320 X (1 litre/1000mL) = 0.320 L solution
Second use the formula = M x V
= 5.0 moles glucose/ litre solution X 0.320 L solution
= 1.6 moles of glucose present in 320mL of solution
8) Explain what is the difference between fractionation and distillation?
Both methods are used to separate the components present in the solution based on the boiling points.
- Distillation: This technique is used when the boiling point of chemicals are different in the mixtures
- Fractionation: This technique is used when the boiling point of chemicals are close to each other in the mixtures
9) Mention the formula to calculate pH of a solution?
In order to calculate the pH of a solution you have to use the formula pH= -log [H+] or pH = -log [H3O+]
10) What is the difference between Molarity and Normality?
Both techniques are used to the amount of chemical present in the solution. However, they are almost similar but differs in
Molarity | Normality |
Molarity is used to know the total amount of molecules in a 1-liter solution | Normality is used to know the total number of reactive units in 1 liter of solution |
It is expressed as moles of a compound per litre of solution | It is expressed in equivalent per litre |
11) What is Valency?
Valency is a property of groups or atoms, equal to the number of atoms of hydrogen that the group or atom could combine with or displace it in forming compounds.

12) Define Avogadro’s law?
According to Avogadro’s law, at the same temperature and pressure, an equal volume of gases contains the same number of molecules regardless of the chemical nature and physical properties.
Avogadro’s number = 6.023 X 1023
13) Explain what makes a molecule into organic molecule?
In a molecule when a hydrogen atom is less than the ratio of the carbon atom, then such molecules are referred to as an organic molecule.

14) Which metal is used to extract copper from the solution of copper sulfate?
Fe or ferrous is the metal that is used to extract copper from the solution of copper sulphate.
15) Explain what is the chemical composition of fat in the human body?
The fat found in the human body is mainly composed of
- Glycerides
- Glycerides+Phospholipids
- Glycolipids
- Phosphoinositides
- Tocopherol
16) What is the monomer of polyethylene?
The monomer of polyethylene is ethylene
17) What is the formula you will use to calculate how many milliliters of 5.5 M NaOH are required to prepare 400 mL of 1.5M NaOH?
To know the amount or volume of NaOH to prepare 400 mL of 1.5 M NaOH, we use formula
M1 X V1 = M2 X V2
V1 = M2 X V2/ M1
But before that we will convert 400 mL into litre = 0.4 L
5.5 X V1 = 1.5 M x 0.4 L
V1 = 1.5 M X 0.4L/ 5.5
V1= 0.10 L
V1 = 100mL
So, you need 100mL of 5.5 NaOH
18) Why graphite rod is used in nuclear reactor?
A graphite rod is used in a nuclear reactor allows un-enriched uranium to be used as a nuclear fuel.
19) Mention how many milliliters is equal to 1 liter and how many microliters is equal to liter?
- 1 millilitre = 0.001 litre
- 1 microliter = 0.000001 litre
20) What is oxidation and reduction reaction?
- Oxidation = When there is a loss of hydrogen or electrons OR gain of oxygen is known as Oxidation reaction.
- Reduction = When there is a gain of hydrogen or electron OR loss of oxygen is known as a reduction reaction
Example of oxidation-reduction reaction is observed in the human body when an electron is transferred into the cell and oxidation of glucose take place from which we get the energy.
21) What does iron ore consist of?
Iron ore is consists of Fe2O3
22) Explain what is dextro-rotatory and levorotatory?
Levorotation and Dextrorotation are referred to the properties of plane polarized light when light rotates clockwise when it approaches the observer is then known as dextro-rotation, and when the light rotates anti-clockwise then it is referred as levorotation.
A compound which exhibits a dextro-rotation is referred to as dextro-rotatory and which exhibits levorotation is referred to as levorotatory.
These interview questions will also help in your viva(orals)
Number 19 isn’t correct. 1ml = 0.001L and so on
1milliliter =0.0001
1 millilitre is one 1000th of a litre – therefore 1ml = 0.001L
updated! Thanks for pointing this out
How to prepare solution in ppm
Litre
Avogadro’s no is wrong it is 10^+23
That’s also correct it is 6.023×10^23
Very basically important question answer
Yes it’s right 1ml=0.001L Due to it’s A conversion factor form Milli liters to liters i.e (1/1000 of liter)
How can we determine the boiling points of components in the mixture are far or close to each other?
How can u determine the rectified spirit
96% alcohol + 4% water
what is the modern name of element ”sb” ???
stibnite/antimony
Antimony
By fractional distillation or extractive distillation
By using ditillation metgod first smaller boiling point will come first then tha second comes
Nice question
Practicle questions chemistory
How to make M/10 solution
dont know
Dilute solution with 100ml distilled water
imp question
Thank you
Nice qutions
Usefull questions …thnk u for give this questions..
Pls join ts Grp. Very nice and good
What is range of water
0-14
Very useful questions. thanks
calculate repulsive force between two electron? when the distance between then is 4.10* -15 (four into ten two the power -15?
good in improview the knowledge questions.
What is Balance shell????
Very useful .what is valance shell
a place outside the nucleus where an electron can move around the nucleus
Useful questions. but check your answer on question 19.
updated! Thanks for pointing this out
Please provide more questions
Excellent performance for Interview questions once again inter questions basic level
Nice
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I am chemistry student learn about in chemistry
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A microlitre is 10-^6 of a litre. It should read 0.000001L
Thanks for writing. It is updated.
Nice….. Do you have more questions…??
PLS more interview questions on clinical chemistry
Thnx…..Very important questions
Thnx…..
What is mean by pH
Please provide 3years experience interwe qoustions
I ma bsc chemistry I am completely 2108 batch but freshers student
More important questions and answers plz
Really… Very useful
Usefull questions….tq
What is water properties…?
Plz noted That
If 1 ml = 0.001 litre
Then 1microlitre= 0.001 ml hence it is 0.000001 litre
Thanks for writing. It is updated.
i appritiate it thunks
Is is useful to freshers also
very nice questions
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Sir i have done masters in chemistry but sir i want to clear all basic
In 19th question Maybe 1microliter will be 0.000001
Thanks for pointing out. It is updated.
Give more Question for interview
Hi
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Thank you.
Very important information
Give more questions for interview
I need some more information by giving the definitions with formula
Because is to learn with interest
Please provide more basic questions
Give more questions related with Msc chemistry Syllabus.
Nice questions
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Chemistry definition
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All questions are nice but there is a mistake in 12th question. The value of avagadro no. 6.023×10^23 not 10^-23
Hi,
Thanks for writing. It is reviewed.
Chemistry definition
Study of physical and chemical properties
Question no 8 there is small mistake in ans. Use of distillation and fractionation (1st sentence) .
It is corrected..!!